Nova Suliska
The development of a drug begins with knowing empirical data on the use of a substance or plant by the public, followed by proving the pharmacological effects and safety in test animals (plink test), and clinical trials on humans. The use of test animals in pharmacological experiments is very necessary in creating disease models. Test animals that are often used in pharmacological experiments include rats, mice, rabbits, guinea pigs. The Wistar strain and the Sprague Dawley strain are rat strains that are often used for pharmacological experiments in Indonesia. Currently there are several vendors that provide experimental animals such as PT Biofarma, BPOM, UGM, etc. So far, researchers have used references from journals or text books where the animals used are animals that live abroad, so normal values are needed in test animals such as biochemical and hematological parameter values which can be a reference for testing pharmacodynamic effects. and pre-clinical toxicity tests, especially on animals that live or are available in Indonesia. The aim of this research is to determine the values of biochemical and hematological parameters of Wistar and Sprague Dawley rats from several vendors providing experimental animals. Provision of test animals, namely 10 male and female Wistar rats each from PT Biofarma, UGM, IPB. 20 male and female SD rats each from BPOM. Test animals were first acclimatized in the laboratory for 7 days. Hematological parameters, namely hemoglobin, RBC, WBC, lymphocytes, basophils and biochemical parameters, namely glucose, cholesterol, triglycerides, creatinine, urea, SGOT, SGPT, HDL, LDL, were measured.
determine the values of biochemical and hematological parameters of Wistar and Sprague Dawley rats from several vendors providing experimental animals.
The test results serve as a reference for the profile of normal hematological and biochemical values for Wistar and Sprague Dawley rats